Hitrap q high performance cytiva, 17115401, pack of 5. Press and hold the wireless button until the attention light blinks. Cytiva formerly ge healthcare life sciences 17515601 view more versions of this product. Hitrap q hp 1 ml and 5 ml hitrap sp hp 1 ml and 5 ml hitrap q hp and hitrap sp hp are small, prepacked columns made of biocompatible polypropylene. Hitrap chelating hp hitrap chelating hp is one of a range of prepacked 1 ml and 5 ml columns for metal chelate affinity chromatography. The design of the hitrap 4rodrfq is closely related to the design of the 108 mhz structure of the gsihli linac, the highcharge state injector to the unilac. Hitrap q hp is a strong anion exchange chromatography column for highresolution, smallscale protein purification. Hitrap nhsactivated hp is designed specifically to facilitate this process and is supplied with a. Want pdf complete corporate edition on all your computers.
Search the worlds information, including webpages, images, videos and more. Hitrap sp hp and hitrap q hp columns characteristics 1 median particle size of the cumulative volume distribution. Hitrap deae ff, 1 ml hitrap q ff, 1 ml hitrap q xl, 1ml hitrap anx ff high sub, 1 ml columns. For use with application purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotidedependent enzymes, alpha2macroglobulin, coagulation facto rs and related proteins. Instructions 71700400 as hitrap affinity columns ge healthcare hitrap heparin hp, 1 ml and 5 ml hitrap heparin hp is a prepacked ready to use, column for preparative affinity chromatography. The list of abbreviations related to hp hitrap protein. From 2003 to 2016, hewlettpackard desktop, notebook, tablet, and workstation computers included pdf complete software. Easytofollow instructions are included for fast startup and method optimization. Hitrap sp hp are prepacked, readytouse sp sepharose high performance strong cation exchange columns for highresolution, smallscale protein purification. Hitrap sp hp and hitrap q hp are prepacked, ready to use cation and anion exchange. How to scan with an hp printer hp customer support. Protocols and tips in protein purification the university of sheffield.
The reuse of hitrap protein g hp depends on the nature of the sample and should only be performed with identical monoclonals to prevent crosscontamination. Hitrap q hp are prepacked, readytouse q sepharose high performance strong anion exchange columns for highresolution, smallscale protein purification. This results in a concentration of the target substance and a complete picture of the whole sample. See also affinity chromatography columns and media guide, 18112186. Hewlettpackard does not support windows nt on the pavilion. Hitrap sp hp and hitrap q hp provide fast, reproducible, and easy separations in a. Hitrap heparin hp 5 x 1 ml cytiva, formerly ge healthcare. Use the hp smart app to create a pdf of a scanned document. The sleek form factor of the hp envy or hp pavilion allinones makes an attractive statement in any workspace, while the highperformance hp omen gaming systems take gaming and more to an entirely new level. Jul 31, 2006 the heparin used in ge healthcares hitrap heparin hp columns comes from porcine intestinal mucosa. Hitrap q hp cytiva, 290525, pack of 1 ml sigmaaldrich. Hitrap affinity columns histrap hp, 1 ml and 5 ml histrap hp is a prepacked, readytouse column for the preparative purification of histagged recombinant proteins by immobilized metal affinity chromatography imac using precharged ni sepharose high performance.
Hitrap deae ff, 1 ml hitrap q ff, 1 ml hitrap q xl, 1 ml. Hitrap heparin hp columns from ge healthcare biocompare. For the highest resolution and purity in anion exchange chromatography at g scale. Instructions 71700500 ax hitrap affinity columns ge healthcare hitrap chelating hp, 1 ml and 5 ml hitrap chelating hp is a prepacked ready to use, column for preparative affinity chromatography. Hitrap q hp, 1 ml and 5 ml prepacked columns instructions for use hitrap sp hp and hitrap q hp are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample cleanup of charged biomolecules. Hitrap heparin hp columns are designed for highresolution purification of dnabinding proteins, coagulation factors, and other plasma proteins. The drawback of this strategy is that the binding capacity of the. Hitrap affinity columns are ideal for method optimization or small scale purification of target proteins using wellestablished protocols.
Complete, easytofollow instructions are included for fast startup and method optimization. Hp stands for hitrap protein also high pressure and 1694 more rating. Cytiva formerly ge healthcare life sciences hitrap q. For use with application for method scouting, group separations, sample concentration and sample cleanup of charged biomolecules. Ge healthcare life sciences 17515601 hitrap q ff chromatography column, 5 ml volume pack of 5 price. Operating hitrap q hp and hitrap sp hp using hitrap q hp and hitrap sp hp prepacked columns is simple. Convenient and reproducible for fast, easy, highperformance separations. Hitrap q ff is prepacked with q sepharose fast flow, and is a strong anion exchanger for smallscale protein purifications as well as screening of binding and elutions conditions. If a p1pump is used a max flow rate of 1 to 3 mlmin can be run on a hitrap 1 ml column.
Whether you use a syringe and the provided luer adaptor fig 2, a peristaltic pump, or a chromatography system such as akta or fplc system, operation. Fast, simple, and easy separations are provided by the combination of the prepacked column and a highperformance affinity medium. Hitrap q ff is a strong anion exchange chromatography column for smallscale protein purification. All necessary connectors are included for connection to different systems as well as to a laboratory pump and a. Hitrap q hp anion exchange chromatography column cytiva. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. Hitrap chelating hp is particularly suitable for the isolation and. Hitrap q hp is a strong anion exchange chromatography column for high resolution, smallscale protein purification. Affinity media can be prepared by coupling a ligand to a selected gel matrix. It is stable to all commonly used buffers, solutions of urea 8 m, guanidine. The small particle size 34m of the medium allows fast adsorption and desorption even at high sample loadings and flow rates. Looking for online definition of hitrap or what hitrap stands for. The principal programming language for these systems has always been a form of interactive basic, now evolved to a high level of specialization for con trol of automatic test systems and called ats basic.
Instructions 71715400 ak hitrap desalting columns ge healthcare hitrap desalting hitraptm desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i. Data file 18117288 ab ion exhange chromatography q. Hitrap deae ff, 1 ml hitrap q ff, 1 ml hitrap q xl, 1 ml hitrap anx ff high sub, 1 ml sample. Cytiva life sciences hitrap q sepharose fast flow iex columns are strong anion exchangers for rapid separations either alone or connected in series.
With akta system use hitrap deae ff or hitrapq hp cartridges, just screw 2 to 4 of them together if. Troubleshoot pdf and print problems with quickbook. Instructions 71714900 an hitrap ion exchange columns ge healthcare hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml hitrap sp hp and hitrap q hp are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample cleanup of charged biomolecules. To achieve the goal of complete protein purification in one day you should move fast and. Q, sp, deae, and cm sepharose fast flow are based on a robust, 6% highly crosslinked beaded agarose matrix with good flow properties and high loading. Whether you use a syringe and the provided luer adaptor fig. The columns can be operated with a syringe, peristaltic pump or liquid. Cytivahitrap capto iex selection kit hitrap capto iex. Hp atlas is a new compiling system designed to run on hp 9500 series automatic test systems. Histrap hp, 1 ml and 5 ml histrap hp is a ready to use column, prepacked with precharged ni sepharose high performance.
Sp sepharose high performance is a strong cation exchange medium and q sepharose high performance is a strong anion. Prepacked columns for convenient and fast separations. Cytiva formerly ge healthcare life sciences hitrap butyl. Dec 21, 2019 hitrap column format is convenient for use with a syringe, a peristaltic pump, or desaltlng chromatography system. The separation is not affected by the flow rate, in the range 1. Hitrap blue hp, 1 ml and 5 ml hitrap blue hp is a prepacked ready to use, column for preparative affinity chromatography. Sp sepharose high performance is a strong cation exchange medium and q sepharose high performance is a strong. Packed with q sepharose high performance strong quaternary ammonium anion exchange resin. Hitrap nhsactivated hp is designed specifically to facilitate this process and is supplied with a recommended coupling procedure for coupling primary amines. Feb 08, 2021 errors the print and pdf repair tool can fix. Suitable for purification of antithrombin iii, coagulation factors, and other plasma proteins. The low a q allows a short structure of 127 cells that is only 1.
The interaction between the protein and the ligand has been suggested to result from the combined electron donating and acceptingaction of the ligand in a mixed mode hydrophilichydrophobic interaction. We are proud to be part of delivering pdf solutions to hewlettpackard customers. Cytiva hitrap butyl hp columns are used for smallscale protein purification as well as for screening of binding and elution conditions. Fraction d from the histrap hp column was essentially homogeneous. Cytiva formerly ge healthcare life sciences 28411005. It can also be used for publishing documents created in. Press and hold the wireless button on the back of the printer until the edge lighting flashes blue. The special design of the column, together with the medium, provide fast, simple and easy separations in a convenient format. For deae and q sepharose fast flow, the starting buffer must be at least one ph unit above the pi of the molecule to be bound. Histrap hp is a ready to use column, prepacked with precharged ni sepharose. Normally we use deaesepharose ff and sometimes q sepharose ff columns.
The ionic strength should be kept as low as possible to allow all components to bind to the ionic exchange complete picture of the whole sample. Data file 181478 af affinity chromatography hitrap. Within two hours, click the plus sign in the hp smart app to add the printer and set it up on your wireless network. The column is delivered with a stopper on the inlet and a snapoff end on the outlet. Performing a separation with hitrap igm purifcation hp. The complete screening procedure was achieved in only a few hours, including the time for. Consists of five hitrap columns, prepacked with capto ion exchange chromatography media with different characteristics.
Pdf complete is a handy application that comes preinstalled on the hewlett packard devices in order to facilitate access to the pdf files. Chapter 2 starting out with hp 5 to help you get started, hp provides simple solutions for discovering your software, learning windows 95, getting support, and using the internet. The special design of the column, together with the high performance. If a p1pump is used a max flow rate of 1 to 3 mlmin can be run on a hitrap 1 ml column packed with sepharose high performance. Cm sepharose fast flow deae sepharose fast flow q sepharose. For cm and sp sepharose fast flow, the starting buffer must be at least one ph unit below the pi of the molecule to be bound. Hitrap desalting can be used with aqueous solutions in the ph range 2. Separation of protein using a strong anion exchange medium prepacked in the hitrap column format. Hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml vwr. If your printer is found, follow the instructions to complete the setup if your printer does not display or to add another printer to the app, click the plus sign, and then follow the instructions to add the printer if your printer is not found, continue to the next step restore the network defaults from the printer to place it into firsttime.
Hitrap is listed in the worlds largest and most authoritative dictionary database of abbreviations and acronyms the free dictionary. Using hitrap q hp and hitrap sp hp prepacked columns is simple. From allinones, high performance, and gaming pcs to those for business or home use, theres a desktop pc for just about any environment. The drawback of this strategy is that the binding capacity. Manual media selection, method development and optimization. Hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml. In this page you find schematic, users and instructions manuals, service manuals, technical supplement, leaf leads and other good stuff. Download this app from microsoft store for windows 10, windows 8.
Select one of the following tiles from the app home screen to start the scan. Q sepharose high performance sp sepharose high performance. Ion exchange chromatography q sepharose high performance sp. Flow and sepharose xl media in hitrap iex selection kit. Hitrap sp hp and hitrap sp ff 12 index of references3 ref 18115683 edition ab, 200204 52.
Quickbooks cant complete the current action due to a missing component. Place the document on the printer scanner glass or into the automatic document feeder adf. Hitrap q xl, 1ml hitrap anx ff high sub, 1 ml columns. Hewlettpackard relied on pdf complete to provide an amazing outofbox experience for their customers. Cytiva formerly ge healthcare life sciences hitrap butyl hp. Cytiva formerly ge healthcare life sciences 28411005 view more versions of this product. The heparin is covalently coupled to highly crosslinked agarose beads then packed into polypropylene which is noninteractive with biomolecules columns.
1348 607 1777 347 1321 1079 171 551 1394 764 1277 1566 1312 691 1225 1630 407 89 325 827 1691 670 48 36