If using sdspage reducing sample buffer in a heated elution, install the kingfisher flex or 96 heating block see manual for proper installation to heat. Thermo scientific kingfisher flex user manual thermo fisher scientific. We have 1 thermo scientific kingfisher flex manual available for free pdf download. Magmax viral and pathogen nucleic acid isolation kits ruo. User manual for use on kingfisher 96 and kingfisher flex, thermo fisher scientific ref 7444050x0 stratec molecular gmbh, d125 berlin for automated purification of bacterial and viral dna as well as viral rna from human and animal serum and plasma samples, cellfree body fluids, swabs rinse liquid. Using patented magnetic particle separation technology, the kingfisher flex provides a fast and easy way to get high yields of purified samples with excellent reproducibility and quality. Automated trizol rna extraction without phase separation on. The complete user manuals for the kingfisher flex instrument and bindit software are provided on. The kingfisher flex series enables highly versatile, automated magnetic particle processing for dna, rna, protein, or cell purification from a wide array of sources, including blood, cell cultures, tissue lysates, soil, and feces.
Oem thermo scientific kingfisher flex 24 deepwell head magnetic 90day warranty. Thermo scientific kingfisher flex with 96well magnet head thermo scientific multidrop 384 liquid dispenser microplates beads kingfisher 96 kf plates 200 l tracpack magnetic streptavidincoated beads step time min temp oc volume l capture 30 rt 50100 wash 1 x 5 rt 100150 elution 20 37 10 5 eluents are analyzed with a dna. Thermo scientific kingfisher flex manuals manualslib. N174 thermo scientific kingfisher 96 user manual cat. Home lab equipment parts dnarna purification instruments thermo scientific kingfisher flex.
Kingfisher instruments utilize disposable plastic plates and tip combs made of polypropylene, which are ideal for magnetic particle processing due to their low binding affinity for biomolecules. Thermo scientific kingfisher flex thermo scientific kingfisher ml thermo scientific kingfisher processing volume 96. Thermo scientific kingfisher flex about this manual the information in this manual is provided to assist owners, page 1528. Seramag speedbeads magnetic protein agparticles protocol. Pureprep pathogens is compatible with kingfisher flex magnetic particle processor by thermo scientific. Use of this web site constitutes acceptance of the labx user agreement. In stock qiagen thermo scientific biosprint 96 kingfisher 710 magnetic particle processor.
It aims to give you a short introduction on installing the instrument and thermo scientific bindit software as well as starting a purification protocol from the internal software. Thermo scientific kingfisher flex purification system. Combined with the intuitive and user friendly bindit. N12420, or the thermo scientific kingfisher ml user manual cat.
The following protocol is for general use with the kingfisher flex or kingfisher 96 instrument. A complete range of thermo scientific manual and electronic single and multichannel pipettes and tips. Thermo scientific kingfisher flex and kingfisher duo instruments. Service manual thermo scientific kingfisher flex service manual 228 pages brand. Thermo kingfishermagnetic particle processors model 710. How to unbox the kingfisher flex scientific videos thermo. The user will need the kingfisher flex or kingfisher duo magnetic particle. Kingfisher flex with 96 pcr head, 100240 v 5400610 kingfisher flex with kf head, 100240 v 5400620.
Thermo scientific bindit software for kingfisher instruments. Bindit software for kingfisher flex system user manual and software dvd. N07669 thermo scientific kingfisher ml user manual cat. The study was performed in the facilities of thermo fisher scientific oy, vantaa. Abhishek futane scientific officer krsnaa diagnostics. Thermo scientific the kingfisher flex series enables highly versatile, automated magnetic particle processing for dna, rna, protein, or cell purification from a wide array of sources, including blood, cell cultures, tissue lysates, soil, and feces. N07669, the thermo scientific kingfisher duo user manual cat. Kit is compatible with all kingfisher instruments flex, duo prime, and presto and can also be used for manual processing in 96well plate. View application notes demonstrating automation solutions for nucleic acid purification on thermo fisher kingfisher flex instrument. Schedule a kingfisher flex demo ordering information.
Related products for plate setup to help save time and increase reproducibility with the magmax viralpathogen nucleic acid isolation kits, combine with e1cliptip equalizer electronic pipettes and. Preparation of the kingfisher instrument and plate setup note. Thermo scientfic plate pp 96 vbtm 2ml ster cs50 95040460. Kingfisher flex thermo fisher scientific us the kingfisher flex is the most versatile benchtop automated extraction instrument in the lab for 24 or 96 samples per run. Consistent highthroughput extraction and purification of dna, rna, protein, and cells. N16621 thermo scientific kingfisher flex user manual cat. Safe and easy the easytouse kingfisher system and straightforward thermo scientific bindit software protocols facilitate safe. The kit is designed for high throughput laboratories, andor for laboratories requiring a less handson time protocol. This unit is certified by the undersigned to be free of radioactive contamination. Thermo scientfic microtiter 96dw vbtm 2ml cs50 95040450. Application highcontent gene expression analysis with. Appendix a certificate of decontamination the signature of a radiation safety officer is also required when the unit has been used with radioactive materials.
Overall yield g and sample purity a260280, a260230 were determined by thermo scientific nanodrop 2000 uv vis spectrophotometer. Find information on thermo fisher connect analysis apps, instrument management, data storage and security, and collaboration tools. User manual kingfisher flex process with 96 deep well manualzz. Pierce streptavidin magnetic beads are validated and optimized for use with highthroughput magnetic platforms, such as the thermo scientific kingfisher 96 and kingfisher flex instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand. If this order is for an out of service unit, please call service support at 8004384851 to place your order. Thermo scientific microtiter deep well plates, kingfisher 96 kf plates, and kingfisher flex 24 deep well plates are all specifically designed for the kingfisher flex. Thermo scientific kingfisher duo prime brief user manual cat. Taqpath covid19 combo kit and taqpath covid19 combo kit. The kit is also suitable for the kingfisher flex with a 24 deep well head or the kingfisher duo with a 6pin head and a sample volume of 3 ml. Kingfisher flex system thermo fisher scientific youtube.
New the kingfisher flex is the most versatile benchtop automated extraction instrument in the lab for 24 or 96 samples per run for consistent highthroughput extraction and purification of dna, rna, protein, and cells. For more detailed instructions, see the kingfisher flex user manual. Thermo fisher scientific thermo scientific kingfisher flex user manual 3 about this user manual this user manual is written for the actual end user, for example, research scientist or laboratory technician, and provides information on the thermo scientific kingfisher flex, including the installation and operating instructions. Nucleic acid purification workflow optimized fisher scientific. Taqpath covid19 combo kit and taqpath covid19 combo. How to unbox the kingfisher flex thermo fisher scientific. The entire processing time on the kingfisher flex is approximately 60 minutes.
See kingfisher flex or kingfisher 96 user manual for detailed instructions. Automated immunoprecipitation in 40 min using dynabeads and. Safe and easy the easytouse kingfisher system and straightforward. Safe and easy the easytouse kingfisher system and straightforward thermo scientific bindit software protocols facilitate safe sample handling. Minimized handson time high sample purity and yields are obtained with minimal user input, and the maximized walkaway time ensures efficiency. Thermo scientific kingfisher flex manuals manuals and user guides for thermo scientific kingfisher flex. Modify the protocol as needed using the thermo scientific bindit software provided with the instrument. Acid isolation kit with the kingfisher flex with 96 deepwell head instrument, the standard procedure in the user manual provided by the kit manufacturer, thermo fisher, is followed. Controller manual for year 1990thermo fisher scientific always follow basic safety precautions when using the kingfisher flex to reduce the risk of injury, biohazardous contamination, fire, or electric shock. Thermo scientific kingfisher flex service manual pdf download.
The thermo scientific kingfisher flex magnetic particle processor offers great flexibility for highthroughput purification of dnarna, proteins and cells from virtually any source. Before beginning the protocol for purification of nucleic acids, carefully read through the thermo scientific kingfisher flex user manual cat. Thermo scientific kingfisher flex from thermo fisher. Thermo kingfisher magnetic particle processors model 710 96 well thermo scientific kingfisher flex system offers highly versatile, automated magnetic particle processing for dnarna, protein or cell purification from virtually any source. This brief user manual is for the kingfisher flex instrument. Minimized handson time high sample purity and yields are obtained with minimal user input, and the maximized walkaway time ensures ef. Thermo scientific kingfisher purification systems, now feature dedicated thermo scientific kingfisher kits for nucleic acid purification and deliver unparalleled purification in an optimized workflow. Kingfisher flex 96 tip comb for deep well magnets thermo scientific product. Automated trizol rna extraction without phase separation. Thermo scientfic microtiter 96dw vbtm 2ml cs50 95040450 free. Thermo scientific kingfisher flex thermo scientific kingfisher duo prime perkin elmer chemagic 360 nucleic acid extractor 2 rt pcr. Preface safety and special notices ii thermo scientific bindit software for kingfisher instruments user manual thermo scientific thermo scientific kingfisher presto user manual cat. Thermo scientific kingfisher plastics for 96 standard.
Combine antigen sample with 10 g of biotinylated antibody per sample. Read this user manual in its entirety prior to operating the instrument. Abhishek futane scientific officer krsnaa diagnostics pvt. The highlights of the directzol chemistry lends itself to simple integration and automation on the kingfisher flex thermo fisher scientific. User manual for use on kingfisher 96 and kingfisher flex, thermo fisher scientific ref7441050x0 stratec molecular gmbh, d125 berlin for automated purification of viral dna and rna from up to 200 l serum, plasma, cell culture supernatant and other cellfree body fluids, biopsy samples and swab with magnetic beads. N07669 or the thermo scientific kingfisher duo user manual cat. The instrument can process up to 96 samples per run using magnetic rods that transfer beads from plate. Prepare samples efficiently from a variety of materials with the thermo scientific kingfisher flex purification system, which offers highly versatile, automated magneticparticle processing for dnarna, protein or cell purification from virtually any source. Optimize your performance using thermo scientific kingfisher plastics designed specifically for use with the kingfisher flex, duo prime, presto and apex instruments.
Thermo scientific kingfisher flex service manual pdf. Extraction instrument kingfisher flex medical equipment. Kingfisher flex magnetic particle processors, thermo scientific. Service manual thermo scientific kingfisher flex service manual 228 pages. Kingfisher flex magnetic particle processor with 96 kf head thermo scientific 4. Dna extraction, kingfisher flex, nextgeneration seque. Review data for use of the kingfisher flex instrument for automated rna and dna isolation from cells and tissues, blood and body fluids, plants and fungi, and viruses. Protocols and consumables are available on request. Prepare samples efficiently from a variety of materials with thermo scientific kingfisher flex purification systems, which offers highly versatile, automated magneticparticle processing for dnarna, protein or cell purification from virtually any source. Comparison of automated kingfisher flextm purification. Thermo scientific microtiter deep well plates, kingfisher 96 kf plates, and kingfisher flex 24 deep well plates are all specifically designed for the kingfisher flex instruments. Thermo scientific hispur ninta magnetic beads deliver consistent yield. N12420 thermo scientific kingfisher duo prime user manual cat. N12420, and the thermo scientific bindit software for kingfisher instruments version 3.
Kingfisher flex magnetic particle processors, thermo. These plates are designed to enable efficient processing and mixing of magnetic particles, and will significantly improve both the yield and quality of isolated dnarna. Oem thermo scientific kingfisher flex 96 deepwell head magnetic 90day warranty. Please contact your thermo scientific representative for specific kingfisher flex and. Thermo scientific kingfisher nucleic acid purification.
For ip, kingfisher instruments with dynabeads offer the best balance of high yield. Fisher 96 kf plates and kingfisher flex 24 deep well. Combine the 96 deep well tip comb and the kingfisher 96 plate. Guiding you through the process to safely unbox your kingfisher flex instrument. The kingfisher flex is the most versatile benchtop automated extraction instrument in the lab for 24 or 96 samples per run. A complete range of thermo scientific manual and electronic. Parts for kingfisher flex from thermo scientific brand dnarna purification instruments offered by unity lab services, part of thermo fisher scientific. Thermo scientific kingfisher magnetic particle processors cat. Knowledge baseproduct faqs find selfhelp for questions about laboratory and industryspecific instruments and equipment, life science research products and data analysis, and informatics products. Before beginning the dna purification protocol, carefully read through the thermo scientific kingfisher flex user manual cat.
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